Optimization of Conditions for Extracting Crude Polysaccharide from Hygrophorus russula

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The nature of the starting material (i.e. powdered versus chipped fruit bodies of Hygrophorus russula) and increasing extraction frequencies were compared using polysaccharide yields as an index of extraction efficiency. Higher polysaccharide yields were obtained when extractions were carried out using powdered H. russula fruit bodies compared with fruit body chips, and crude polysaccharide yields of almost 4.3% were obtained from powdered material after two consecutive extractions using distilled water. Application of orthogonal testing to optimize extraction conditions revealed that polysaccharide yields were influenced mainly by the ethanol concentration used for precipitation, followed by temperature, solid:liquid ratio and treatment time. The optimal conditions were: extraction time 4 h, temperature 80 ℃, solid:liquid ratio 1:10, and ethanol concentration 85%. Under these conditions, a polysaccharide yield of 6.52% was obtained. The nature of the starting material (ie powdered versus chipped fruit bodies of Hygrophorus russula) and increasing extraction frequency were compared using polysaccharide yield as an index of extraction efficiency. Compared with fruit body chips, and crude polysaccharide yields of substantially equal to 4.3% were obtained from powdered material after two consecutive extractions using distilled water. Application of orthogonal testing to optimize extraction conditions that said polysaccharide yields were mainly mainly by the ethanol concentration used for precipitation, followed by temperature, solid: liquid ratio and treatment time. The optimal conditions were: extraction time 4 h, temperature 80 ° C, solid: liquid ratio 1:10, and ethanol concentration 85% was obtained.
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