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目的:检测水通道蛋白5(AQP5)、缺氧诱导因子1α(HIF-1α)和血管内皮生长因子(VEGF)在鼻息肉组织中的表达情况,并探讨3种因子之间表达的相关性及其在鼻息肉发病机制中的作用。方法:取18例患者鼻息肉组织(鼻息肉组)及10例单纯行鼻中隔偏曲矫正术中切除的下鼻甲组织(对照组),分别运用RT-PCR和Western blot检测AQP5、HIF-1α、VEGF mRNA以及蛋白的表达情况;采用t检验和直线相关分析进行统计学分析。结果:①RT-PCR结果显示,AQP5 mRNA在鼻息肉组中的表达明显低于对照组,差异有统计学意义(P<0.01),HIF-1α、VEGF mRNA在鼻息肉组和对照组中表达差异无统计学意义(P>0.05);②Western blot结果显示AQP5蛋白表达鼻息肉组与对照组无明显差异(P>0.05),HIF-1、VEGF蛋白表达水平在鼻息肉组中的表达显著高于对照组(P<0.05);③Western blot结果显示鼻息肉组中AQP5与HIF-1α之间蛋白的表达呈明显正相关(r=0.633,P<0.01),AQP5与VEGF蛋白质表达亦呈明显正相关(r=0.611,P<0.01)。结论:AQP5与HIF-1α、VEGF等低氧反应通路因子共同参与鼻息肉组织水肿的形成。前者通过组织分布异常引起水转运障碍进而导致组织水肿形成,后者主要通过诱发血管增殖、增加血管通透性进而导致组织水肿产生;两者是相对独立的系统,推测其可能通过某种间接途径发生联系。
Objective: To detect the expression of aquaporin 5 (AQP5), hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in nasal polyps and to investigate the correlation between the three factors Its role in the pathogenesis of nasal polyps. Methods: 18 cases of nasal polyps (nasal polyps) and 10 cases of inferior nasal turbinate tissue resected in nasal septum deviation correction were randomly divided into three groups. The expression of AQP5, HIF-1α, VEGF mRNA and protein expression; using t test and linear correlation analysis for statistical analysis. Results: ①RT-PCR results showed that the expression of AQP5 mRNA in nasal polyps group was significantly lower than that in control group (P <0.01), while the expression of HIF-1α and VEGF mRNA in nasal polyps group and control group (P> 0.05). ②Western blot results showed that the expression of AQP5 protein in nasal polyps group was not significantly different from the control group (P> 0.05). The expression of HIF-1 and VEGF protein in nasal polyps group was significantly higher than that in nasal polyps group (P <0.05). ③Western blot results showed that the expression of AQP5 and HIF-1α in nasal polyps was positively correlated (r = 0.633, P <0.01), and the protein expression of AQP5 was also positively correlated (r = 0.611, P <0.01). Conclusion: AQP5 and HIF-1α, VEGF and other hypoxia pathways participate in the formation of nasal polyp edema. The former caused by abnormal tissue distribution caused by water transport obstacles and then lead to the formation of tissue edema, the latter mainly by inducing blood vessel proliferation, increased vascular permeability resulting in tissue edema; both are relatively independent system, suggesting that it may by some indirect pathway Contact occurred.