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通过观察丙型肝炎病毒阳性血清处理后小鼠小胶质细胞BV2(BV2细胞)内TLR7蛋白及TNF-α、IFN-α的表达变化,探讨HCV对中枢神经系统损伤的免疫发病机制。用20%HCV阳性血清处理BV2细胞,在处理后24 h收集细胞及培养上清液,应用间接免疫荧光流式细胞术及酶联免疫吸附法(ELISA)分别检测细胞内TLR7蛋白和培养上清液中TNF-α、IFN-α的表达,同时设同型对照、空白组和正常人血清组。结果表明,BV2细胞内有TLR7蛋白的表达,其中HCV阳性血清组表达明显升高,较空白组及正常人血清组比较具有统计学意义(P<0.05),正常人血清组与空白对照组比较无统计学意义。HCV阳性血清处理BV2细胞24h后TNF-α、IFN-α水平均较空白组及正常人血清组增高,具有统计学意义(P<0.05),正常人血清组与空白对照组比较无统计学意义(P>0.05)。本研究初步阐明了HCV阳性血清处理后BV2细胞中TLR7表达显著升高,TLR7激活后能够启动BV2细胞的固有免疫反应,因此TLR7可能在HCV导致的CNS的发病中发挥重要作用。
By observing the changes of TLR7 protein, TNF-α and IFN-α in microglia BV2 (BV2 cells) treated with hepatitis C virus positive serum, we explored the immune pathogenesis of HCV in central nervous system. BV2 cells were treated with 20% HCV positive serum, and the cells and culture supernatants were collected 24 hours after treatment. Indirect immunofluorescence flow cytometry and enzyme-linked immunosorbent assay (ELISA) were used to detect the intracellular TLR7 protein and culture supernatant Fluid TNF-α, IFN-α expression, while setting the same type of control, blank group and normal human serum group. The results showed that the expression of TLR7 protein in BV2 cells, including HCV positive serum group was significantly higher than the blank group and normal human serum group was statistically significant (P <0.05), normal human serum group compared with the blank control group No statistical significance. The levels of TNF-α and IFN-α in BV2 cells treated with HCV positive serum for 24h were significantly higher than those in blank group and normal human serum group (P <0.05), and there was no significant difference between normal serum group and blank control group (P> 0.05). This study initially clarified the significant increase of TLR7 expression in BV2 cells after treatment with HCV-positive serum. TLR7 activation could activate the innate immune response of BV2 cells. TLR7 may play an important role in the pathogenesis of HCV-induced CNS.