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目的 :观察应用鹿茸后骨折愈合过程中 ,TGF -β1 、BMP -2的表达变化 ,以进一步探讨其促进骨折愈合的机理。方法 :将雄性大鼠 60只随机分为 4组 :生理盐水组A、伤科接骨片组B、鹿茸低剂量组C、鹿茸高剂量组D。按标准模型方法造模 ,不作任何固定 ,分笼饲养 ,术后第 2天灌药 ,于术后 7、14、2 1天分别将每组处死 5只大鼠 ,取出骨标本 ,制成切片。测量骨痂厚度 ,利用免疫组化染色及图像分析测定TGF -β1 、BMP -2表达强度。结果 :在骨折愈合过程中 ,实验组和对照组的骨痂厚度及骨痂组织中TGF -β1 、BMP -2的含量存在着差异 ,实验组和伤科接骨片组的染色峰值出现在第 2周 ,对照组出现在第 3周。结论 :高剂量鹿茸能够增加大鼠骨折端骨痂厚度 ,提高骨折愈合质量 ,并能增加TGF -β1 、BMP -2在骨痂组织中表达 ,可能为鹿茸促进骨折愈合的机制之一
Objective: To observe the expression changes of TGF-β1 and BMP-2 in the process of fracture healing using pilose antler in order to further explore the mechanism of promoting fracture healing. Methods : 60 male rats were randomly divided into 4 groups: saline group A, Shangke Jiegu tablets group B, pilose antler low dose group C, antler high dose group D. Models were made according to the standard model method, without any fixation, caged and fed on the second day after operation. Five rats in each group were sacrificed on the day of 7, 14 and 21 days after operation. Bone samples were taken and sliced. . The thickness of callus was measured and the expression of TGF-β1 and BMP-2 was measured by immunohistochemical staining and image analysis. Results: During fracture healing, the thickness of callus in the experimental group and the control group and the content of TGF-β1 and BMP-2 in the callus were different. The peak of staining in the experimental group and the Shangke Jiegu Tablet group appeared in the second Weeks, the control group appeared in the third week. Conclusion : High dose pilose antler can increase the thickness of fractured callus in rats, improve the quality of fracture healing, and increase the expression of TGF-β1 and BMP-2 in callus. It may be one of the mechanisms of pilose antler to promote fracture healing.