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目的研究外阴阴道炎患儿血浆甘露糖结合凝集素(MBL)水平变化及mbl2外显子1区SNP位点的变异频率。方法血浆MBL浓度的检测采用ELISA法,mbl2SNP位点的多态性分析采用序列分析法。研究对象分为疾病组(102例)和对照组(90例),统计分析采用SPSS 11.0软件,遗传学分析采用SHEsis软件。结果疾病组中,分泌物致病菌分离率为42.7%。对照组与疾病组血浆MBL浓度中位数分别为1 360μg/L和1 064μg/L,疾病组MBL浓度显著低于对照组(Z=2.26,P=0.03)。疾病组+230位点的变异频率为0.196,显著高于对照组中0.111的频率(χ2=5.237,P=0.022)。所有样本外显子1区已知SNP+239位点、+223位点、+101位点、+71位点均无变异发现,外显子1序列全长无新突变发现,AA型外显子血浆MBL水平显著高于AB型,后者又显著高于BB型。结论本地区人群mbl2外显子1区变异以+230位点为主,该位点变异导致的低血浆MBL浓度与患儿外阴阴道炎的发生存在一定的相关关系。
Objective To study the changes of plasma mannose binding agglutinin (MBL) levels and the frequency of SNP loci in exon 1 of mbl2 in children with vulvovaginitis. Methods The plasma concentration of MBL was detected by ELISA. The polymorphism of mbl2 SNP was analyzed by sequence analysis. The subjects were divided into disease group (102 cases) and control group (90 cases). SPSS 11.0 software was used for statistical analysis and SHEsis software was used for genetic analysis. Results In the disease group, the isolation rate of secreted pathogens was 42.7%. The median plasma concentrations of MBL in the control group and the disease group were 1 360 μg / L and 1 064 μg / L, respectively. The MBL concentration in the disease group was significantly lower than that in the control group (Z = 2.26, P = 0.03). The frequency of mutation at +230 site was 0.196 in disease group, which was significantly higher than that of 0.111 in control group (χ2 = 5.237, P = 0.022). No SNP + 239 loci, + 223 loci, + 101 loci, + 71 loci were found in all the exons 1 of the samples. No mutation was found in the exon 1 sequence, Sub-plasma MBL levels were significantly higher than the AB type, the latter was significantly higher than the BB type. Conclusion The variation of exon 1 in mbl2 region in the population of this region is dominated by +230 locus. The low plasma MBL concentration induced by this locus has a certain relationship with the occurrence of vulvovaginitis in children.