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PCR amplification employing a SCAR (Sequence Characterized Amplified Region) primer pair, 135F/R,(amplifying a band of 601 bp) for identifying ’135’ strains of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a L.edodes 135 strain.Protoplast monok.aryons segregated into either A1B1 or A2B2 mating type,and the SCAR marker was detected only in the latter.The marker was also detected in four delineated mating types (A1B1,A2B2,A1B2,A2B1) of spore monokaryons.Our data testify to the stable inheritance of the specific SCAR marker,and show that the SCAR locus is not genetically linked to either the A or B mating factors.
PCR amplification employing a SCAR (Sequence Characterized Amplified Region) primer pair, 135F / R, (amplifying a band of 601 bp) for identifying ’135’ strain of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a L. edodes 135 strain. Protoplast monok.aryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The marker was also detected in four delineated mating types (A1B1, A2B2, A1B2, A2B1) of spore monokaryons. Our data testify to the stable inheritance of the specific SCAR marker, and show that the SCAR locus is not genetically linked to either the A or B mating factors.