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不同关键酶基因在甘蓝型油菜种子发育过程中对油脂积累的影响重大。为此,本实验选取3个不同含油量的甘蓝型油菜作为模型。研究BnACC1、BnDGAT1和BnPDAT1基因在甘蓝型油菜种子发育时期的表达模式。结果表明:BnACC1在这三种甘蓝型油菜种子中的基因表达模式基本一致,在低含油量油菜的种子发育前期和高含油量油菜的种子成熟期相对表达量高,说明在油脂积累旺盛期该基因的高表达可提高含油量;而BnDGAT1和BnPDAT1在这三种甘蓝型油菜表达模式存在差异。其中,BnDGAT1基因在高含油量油菜中的相对表达量高于低含油量油菜。这说明BnDGAT1基因对油脂积累有一定的促进作用。BnPDAT1基因的相对表达量与甘蓝型油菜含油量密切相关。本研究可以为提高油菜含油量的相关分子机制提供了科学依据。
The effects of different key enzyme genes on the lipid accumulation during the seed development of Brassica napus were significant. To this end, the experiment selected three different oil content of Brassica napus as a model. To study the expression patterns of BnACC1, BnDGAT1 and BnPDAT1 genes during the seed development stage of Brassica napus. The results showed that the gene expression patterns of BnACC1 in these three Brassica napus seeds were basically the same. The relative expression level of BnACC1 in the seeds of rapeseed with low oil content and the seed maturity of high oil content rape showed that at the peak of oil accumulation High gene expression increased oil content, while BnDGAT1 and BnPDAT1 differed in the expression pattern of these three Brassica napus. Among them, the relative expression level of BnDGAT1 in high oil content rape was higher than that in low oil content rape. This shows that BnDGAT1 gene has a certain role in promoting lipid accumulation. The relative expression of BnPDAT1 gene is closely related to the oil content of Brassica napus. This study can provide a scientific basis for improving the molecular mechanism of rapeseed oil content.