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应用平滑肌依赖于钙(CaM)的肌球蛋白轻链激酶(MLCK)体系,观察了 Pb~(++)与 MLCK的相互作用,得到以下结果:(1)Pb~(++)在低浓度时激活 MLCK,高浓度时抑制此酶活力;(2)Pb++对 MLCK 的激活作用,被 CaM 拮抗剂三氟啦嗪所抑制;(3)Pb~(++)对 MLCK 的抑制作用在于降低酶的基础活力,即作用于酶的本身;(4)CaM 能对抗 Pb~(++)的抑制,但 Ca~(++)增强 Pb~(++)的抑制;(5)二硫苏糖醇和 EDTA 均能逆转 Pb~(++)对 MLCK 的抑制,并且 EDTA—
The interaction between Pb ++ and MLCK was observed using smooth muscle-dependent calcium (CaM) myosin light chain kinase (MLCK) system. The following results were obtained: (1) Pb ++ at low concentrations When MLCK is activated, the activity of this enzyme is inhibited at high concentrations; (2) The activation of MLCK by Pb++ is inhibited by the CaM antagonist trifluoperazine; (3) The inhibition of MLCK by Pb ++ is to decrease the enzyme The basic activity of the enzyme, namely, the enzyme itself; (4) CaM can inhibit the inhibition of Pb ++, but Ca ++ enhances the inhibition of Pb ++; 5, dithiothreitol Both alcohol and EDTA can reverse Pb ++ inhibition of MLCK, and EDTA-