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目的制备聚电解质逐层组装的壳聚糖海藻酸钠微凝胶,考察体外释放规律,观察体内释放过程。方法使用高压静电成囊机制备海藻酸钠壳聚糖微凝胶核心,通过静电吸附原理将聚电解质逐层包裹在微凝胶表面形成包衣层;考察不同物料比例、包衣层数对体外释药性能的影响;小鼠皮下注射制剂后,制备组织切片观察体内释放过程。结果海藻酸钠浓度、氯化钙浓度、聚电解质包衣层数影响脉冲释放时滞以及时滞后释放速度。结论聚电解质逐层组装的微凝胶脉冲制剂,体外释药性能可灵活调控,体内释药可见,并且制剂安全,生物相容性好。
OBJECTIVE: To prepare chitosan alginate microgels assembled with polyelectrolytes layer by layer, investigate the in vitro release patterns and observe the in vivo release process. Methods The sodium alginate chitosan microgel core was prepared by using high voltage electrostatic capsule machine. The polyelectrolyte was coated on the surface of microgel by electrostatic adsorption principle to form the coating layer. The ratio of different materials and the number of coating layers were measured in vitro Drug release performance; subcutaneously injected mice, the preparation of tissue sections observed in vivo release process. Results Sodium alginate concentration, calcium chloride concentration and polyelectrolyte coating number affected the pulse release delay and the delayed release rate. Conclusion The polyelectrolyte microgel gelled implants can be flexibly controlled in vitro and released in vivo. The preparation is safe and has good biocompatibility.