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在组织学和病理学诊断中,为显示细胞核的结构常用Harris或Ehrlich铝-苏木精溶液,两液均有其不足。Harris液表面有一层金属膜,溶液中沉淀较多,故使用前需过滤;有时出现氢氧化铝凝胶现象;在加氧化剂时,因能产生大量气体,易使溶液从容器内喷出;除细胞核着色外,其它组织成分的复染较重,故必须用酸酒精分化;溶液中的苏木精氧化等级高,因此溶液的使用期较短等缺点。Ehrlich液因不加氧化剂,故不能立即使用,于空气中自然氧化,一般需四周以上因此对其氧化程度难以做到严格控制,其着色能力因配制时间的长短而异,陈液较新液的着色力强,从而不易掌握染色时间,需用盐酸酒精来调节。Gill(1974)按Baker(1953,1960,1962)提出半氧化概念和铝媒染值的建议,配制半氧化苏木精并将haematal-16降为haematal-8的溶液,取
In both histological and pathological diagnosis, both fluids are deficient in order to show that the structure of the nucleus is usually Harris or Ehrlich aluminum-hematoxylin solution. Harris liquid surface has a layer of metal film, precipitation in solution is more, so before use to be filtered; sometimes aluminum hydroxide gel phenomenon; in the addition of oxidants, because of the large amount of gas can be generated, easy to make the solution from the container; In addition to the staining of the nucleus, the complex staining of other tissue components is so heavy that it must be differentiated with acid alcohol; the hematoxylin in the solution is oxidized to a high level and therefore has a short pot life. Ehrlich liquid due to the absence of oxidants, it can not be used immediately, the natural oxidation in the air, usually more than four weeks so the degree of oxidation is difficult to be strictly controlled, its coloring ability due to the length of preparation time varies, Strong tinting strength, which is not easy to grasp the dyeing time, need to use hydrochloric acid to regulate. According to Baker’s (1953, 1960, 1962) proposal for the concept of semi-oxidation and aluminum mordant value, Gill (1974) formulated semi-oxidized hematoxylin and reduced haematal-16 to a solution of haematal-8