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目的 :探讨血管内皮生长因子 (VEGF)和血管内皮生长因子C(VEGF C)在喉鳞癌组织及癌旁组织中的表达及其与喉癌生物学行为之间的关系。方法 :采用逆转录聚合酶链式反应技术 ,对 2 0例新鲜喉鳞癌标本、切缘组织及远离肿瘤的正常喉组织中的VEGF、VEGF C基因表达进行检测。结果 :对同一患者 ,VEGF、VEGF C在肿瘤组织、切缘组织、正常组织中的表达差异有显著性意义 (P <0 .0 5 ) ,在肿瘤组织中 ,VEGF、VEGF C的表达与患者年龄、性别、病变部位、T分期、病理分级无关 ,VEGF、VEGF C表达与喉鳞癌淋巴结转移有关。结论 :VEGF、VEGF C联合检测可望作为判断喉鳞癌患者预后的指标。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor C (VEGF C) in laryngeal squamous cell carcinoma and its adjacent tissues and their relationship with the biological behavior of laryngeal carcinoma. Methods: The expression of VEGF and VEGF C in 20 cases of fresh laryngeal squamous cell carcinoma, the marginal tissues and the normal laryngeal tissues away from the tumor were detected by reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of VEGF and VEGF C in the same patient was significantly different between the tumor tissues, the marginal tissues and the normal tissues (P <0.05). The expression of VEGF and VEGF C in the same patients was significantly higher than that in the patients Age, gender, lesion location, T stage, pathological grade, VEGF, VEGF C expression and lymph node metastasis of laryngeal squamous cell carcinoma. Conclusion: The combined detection of VEGF and VEGF C is expected to be used as an index to judge the prognosis of patients with laryngeal squamous cell carcinoma.