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为了证实重组质粒ＤＮＡ分子中插入的外源ＤＮＡ确是来自变形链球菌拱体菌，检测受体菌中是否有探针同源的序列。本研究采用ＤＮＡＳｏｕｔｈｅｒｎ印迹杂交、点印迹杂交和切口平移制备生物素ＤＮＡ探针以及光敏基因检测技术，对乳链球菌ＨＬ１０２，ＨＬ１０７中的重组质粒ＤＮＡｐＬＦ１０２，ｐＬＦ１０７作了进一步分析鉴定，ＤＮＡ点印迹杂交结果显示，含有ｐａｃ基因的ｐＰＣ４１ＰｓｔⅠ酶切ＤＮＡ探针均与上述质粒ＤＮＡ结合，Ｓｏｕｔｈｅｒｎ印迹杂交提示重组质粒１．５ｋｂ的酶切片段与生物素探针杂交，证实该基因片段是从ｐＰＣ４１中获得的变形链球菌目的基因片段，重组乳链球菌ＨＬ１０２，ＨＬ１０７均携带变形链球菌表面蛋白抗原（ＰＡｃ）结构基因ｐａｃ。
In order to confirm that the exogenous DNA inserted in the recombinant plasmid DNA was indeed from the Streptococcus mutans arches, it was detected whether there was a probe-homologous sequence in the recipient bacteria. In this study, DNASouthern blot hybridization, dot blot hybridization and nick translation of biotin DNA probes and detection of light-sensitive genes, the Streptococcus agalactiae HL102, HL107 recombinant plasmid DNApLF102, pLF107 further analysis and identification, DNA dot blot hybridization results Showed that the pPC41PstI digested DNA probe containing the pac gene was bound to the above-mentioned plasmid DNA, and Southern blotting indicated that the 1.5 kb fragment of the recombinant plasmid was hybridized with the biotin probe to confirm that the gene fragment was transformed from pPC41 Streptococcus target gene fragment, recombinant Streptococcus lactis HL102, HL107 carry Streptococcus mutans surface protein antigen (PAc) structural gene pac.