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用海绵作载体，以甲醛、生理盐水和抗生素为防腐介质，用塑料袋分０．５亿、１．０亿和１．５亿条线虫三种密度包装，在８～１０℃下贮存。以亚洲玉米螟幼虫为试虫，按幼虫与线虫比为１：１２５、１：２５、１：５三个剂量测定贮存线虫的毒力。结果表明，线虫在低温下贮存１年其存活率达８５％以上；贮存介质对贮存线虫的存活率有明显影响，而线虫密度、加水量对其影响不大。贮存５个月以内的线虫对玉米螟幼虫毒力差异不明显。
With sponge as a carrier, formaldehyde, saline and antibiotics as preservative medium, with plastic bags divided into 50 million, 100 million and 150 million nematodes three kinds of density packaging, stored at 8 ~ 10 ℃. The larvae of Asiatic corn borer (A. furnacalis) were used as test insects, and the virulence of stored nematodes was determined according to the larvae and nematode ratios of 1: 125, 1: 25 and 1: 5. The results showed that the survival rate of nematodes stored at low temperature for 1 year was more than 85%. The storage medium had a significant effect on the survival rate of nematode. However, nematode density and water addition had little effect on it. Nematodes stored within 5 months did not show any significant difference in toxicity to corn borer larvae.