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目的探讨木糖醇(Xylitol,Xy)对糖尿病(DM)模型大鼠骨质疏松(osteoporosis,OP)的影响。方法将48只3个月龄、健康、雄性Wistar大鼠随机分为糖尿病对照组(DC组)、糖尿病+10%Xy组(DM+10%Xy组)、糖尿病+20%Xy组(DM+20%Xy组)、健康对照组(NC组)、健康+10%Xy组(N+10%Xy组)、健康+20%Xy组(N+20%Xy组)。前3组以四氧嘧啶、柠檬酸-柠檬酸钠缓冲液制作糖尿病大鼠模型且造模成功。饲料中添加一定比例的Xy喂养,12周后处死。留取血及骨标本测定血清总钙(Ca)、无机磷(P)、碱性磷酸酶(ALP)、骨钙素(BGP)及骨密度(BMD)和骨形态计量学及骨的生物力学指标。结果 DM大鼠出现血糖升高、体重下降、骨量减少、骨组织形态和骨生物力学异常。低、高剂量Xy降低DM大鼠血糖,增加骨量、骨强度,改善骨组织形态和骨生物力学特性,对预防OP有一定作用;但低剂量组作用不明显,此作用可能存在剂量依赖关系。Xy未降低健康大鼠血糖,但可直接作用于骨,增加骨矿物质含量。结论 DM大鼠出现骨形成、骨转换和骨生物力学异常,木糖醇可降低DM大鼠血糖,对其骨量、骨组织形态及骨生物力学产生影响,此作用可能存在剂量依赖关系。
Objective To investigate the effect of Xylitol (Xy) on osteoporosis (OP) in diabetic rats. Methods 48 Wistar rats of 3 months old, healthy and male were randomly divided into diabetic group (DM group), DM + 10% Xy group (DM + 10% Xy group) and DM + 20% Xy group), healthy control group (NC group), healthy + 10% Xy group (N + 10% Xy group) and healthy + 20% Xy group (N + 20% Xy group). The first 3 groups of alloxan, citric acid - sodium citrate buffer model of diabetic rats and modeling success. Feed a certain percentage of Xy feeding, sacrificed 12 weeks later. Blood and bone samples were collected for determination of serum total calcium (Ca), inorganic phosphorus (P), alkaline phosphatase (ALP), osteocalcin (BGP) and bone mineral density (BMD), bone morphometry and biomechanics index. Results The hyperglycemia, weight loss, osteopenia, bone histomorphology and biomechanical abnormalities were observed in DM rats. Low and high doses of Xy could decrease the blood glucose, increase the bone mass, bone strength and improve the bone histomorphology and biomechanical properties of DM rats, which may play a role in the prevention of OP. However, the effect of low dose and high dose of Xy is not obvious, which may be dose dependent . Xy did not reduce blood glucose in healthy rats, but could directly affect the bone and increase the bone mineral content. Conclusion The bone formation, bone turnover and bone biomechanics in DM rats are abnormal. Xylitol can reduce the blood glucose in DM rats, which may affect the bone mass, bone morphology and biomechanics. This effect may have a dose-dependent relationship.