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目的探讨卡介苗(BCG)诱导小鼠脾脏树突状细胞分化作用。方法分别用PBS和BCG免疫小鼠,收集免疫鼠脾单核细胞培养,通过瑞士-姬姆萨染色观察脾单核细胞形态;流式细胞术分析脾巨噬细胞、树突状细胞表型;MTS法检测小鼠脾淋巴细胞增殖;ELISPOT法检测脾淋巴细胞IFN-γ的分泌;ELISA法测定小鼠脾单核细胞分泌IL-2情况。结果与对照组相比,BCG免疫组镜下可见体积大、带毛刺状突起的细胞较多;CD14(P<0.05)、CD40、CD11C、CD86、CD68表达水平增高;小鼠脾淋巴细胞刺激指数增高(P<0.05);分泌IFN-γ的细胞频数增多(P<0.01);脾单核细胞培养上清中IL-2水平明显增高(P<0.01)。结论 BCG可诱导小鼠树突状细胞分化并活化Th1细胞。
Objective To investigate the differentiation of dendritic cells induced by BCG in mice spleen. Methods Mice were immunized with PBS and BCG respectively. The spleen mononuclear cells were harvested and the morphology of spleen mononuclear cells was observed by Swiss-Giemsa staining. The phenotypes of splenic macrophages and dendritic cells were analyzed by flow cytometry. The MTS method was used to detect the proliferation of mouse splenic lymphocytes; the secretion of IFN-γ by spleen lymphocytes was detected by ELISPOT; the secretion of IL-2 by spleen mononuclear cells was measured by ELISA. Results Compared with the control group, there were more bulky cells with burr-like protrusions in BCG immunohistochemistry. The expression of CD40, CD11C, CD86 and CD68 were increased in CD14 (P <0.05), and the splenic lymphocyte stimulation index (P <0.05). The frequency of IFN-γ-secreting cells increased (P <0.01), and the level of IL-2 in supernatant of splenic monocytes increased significantly (P <0.01). Conclusion BCG can induce dendritic cell differentiation and activate Th1 cells in mice.