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目的：评价维拉帕米（ｖｅｒａｐａｍｉｌ，Ｖｅｒ）对人巩膜成纤维细胞（ｈｕｍａｎｓｃｌｅｒａｌｆｉｂｒｏｂｌａｓｔｓ，ＨＳＦ）增殖的影响。方法：培养的ＨＳＦ分别加入不同浓度的Ｖｅｒ及５－ＦＵ、肝素（ｈｅｐａｒｉｎ，Ｈｅｐ）、地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，Ｄｅｘ），用四甲基偶氮唑盐（ＭＴＴ）比色法，在酶联免疫测定仪上测５４０ｎｍ处吸光值，计算出各药物浓度条件下的净增抑制率。实验数据行ｔ检验。结果：Ｖｅｒ在２０ｍｇ／Ｌ以上使细胞增殖率显著下降；６０ｍｇ／Ｌ以上使部分细胞生存受到影响。将Ｖｅｒ分别与不同浓度的５－ＦＵ、Ｈｅｐ、Ｄｅｘ合用，抗增殖作用明显增强。结论：Ｖｅｒ对ＨＳＦ增殖有抑制作用，并能增强５－ＦＵ、Ｈｅｐ、Ｄｅｘ的抗增殖作用效果
Objective: To evaluate the effect of verapamil (Ver) on the proliferation of human scleral fibroblasts (HSF). Methods: Cultured HSF were added with different concentrations of Ver and 5-FU, heparin (Hep), dexamethasone (Dex) and MTT colorimetric method respectively. Measured at 540nm absorbance measured on an immunoassay to calculate the net increase inhibition rate of each drug concentration conditions. Experimental data line t test. Results: Ver at 20mg / L or more significantly reduced cell proliferation rate; 60mg / L above the part of cell survival affected. The combination of Ver with 5-FU, Hep and Dex in different concentrations significantly enhanced the antiproliferative effect. Conclusion: Ver has an inhibitory effect on the proliferation of HSF and enhances the antiproliferative effects of 5-FU, Hep and Dex