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作者为了观察电离辐射的直接效应,应用Yamada等人设计的体外受精卵培养法,主要以X线照射体外精子后,受精卵的第一次分裂中期染色体畸变为指标。以2～4月龄的(C57BL/6J×C3H/He)F_1雌、鼠及3～5月龄的RFM雄鼠为对象进行了实验研究。在给雌鼠腹膜内注射7.5IU孕马血清促性腺激素(PMSG)后的48小时,再注射7.5IU人绒毛膜促性腺激素(hCG),待15小时后,从输卵管获取卵子。从雄鼠附睾中获取精子,并在体外用X线照射成熟精子。照射条件:250kVp西门子X线机,60R/分,照射剂量分别为50、100、200、400R。将X线照射过的精子悬液(精子浓度200个/mm~3)直接加到含卵的培养基内,孵
In order to observe the direct effect of ionizing radiation, the in vitro fertilized egg culture method designed by Yamada et al. Was used as an index, mainly on the first metaphase chromosome aberration of fertilized eggs after X-ray irradiation on in vitro sperm. The experimental studies were conducted on 2 to 4 months old (C57BL / 6J × C3H / He) F_1 female mice and RFM male mice aged 3 to 5 months. Forty-eight hours after the mice were injected intraperitoneally with 7.5 IU of pregnant horse serum gonadotropin (PMSG), 7.5 IU human chorionic gonadotropin (hCG) was injected, and after 15 hours eggs were taken from the fallopian tubes. Sperm were obtained from the male rat epididymis and were irradiated with X-rays to mature sperm in vitro. Irradiation conditions: 250kVp Siemens X-ray machine, 60R / min, irradiation doses were 50,100,200,400 R. X-ray irradiation of the sperm suspension (sperm concentration of 200 / mm ~ 3) directly into the egg-containing medium, incubation