论文部分内容阅读
采用UPGMA法对11个甘薯品种(系)不同农艺性状进行聚类分析。在相似系数为0.17处被划分为3大类群,分别包括2、3、6个品种。SRAP聚类分析表明:在相似系数为0.77处,第Ⅱ类群划分为2个亚类:ⅡA和ⅡB,ⅡA有3个品种,ⅡB有3个品种;在相似系数为0.75处,第Ⅲ类群划分为2个亚类:ⅢA和ⅢB,ⅢA有2个品种,ⅢB有2个品种。丰收白野生型与突变体M-0在农艺性状以及SRAP分子标记的聚类分析中均聚为一类。对丰收白野生型及其突变体M-0的叶型、叶脉颜色、新生叶色、薯皮色、薯形及肉色6个生物学性状进行观察比较,结果表明二者在前5个性状均未表现出明显差别,只在薯色性状上表现明显差异,即丰收白的薯肉色为白色而突变体M-0为粉红色。丰收白突变体M-0和野生型多态性条带比率为29.49%,有效等位基因数为1.2085,说明二者在遗传上差异不明显,并且初步推断丰收白M-0是丰收白的薯肉色基因发生点突变的结果。
The UPGMA method was used to cluster the different agronomic traits of 11 sweet potato varieties (lines). At the similarity coefficient of 0.17, it was divided into three groups, including 2, 3 and 6 varieties respectively. SRAP clustering analysis showed that at the similarity coefficient of 0.77, the second group was divided into two subgroups: ⅡA and ⅡB, ⅡA three varieties, ⅡB three varieties; at the similarity coefficient of 0.75, the third group division There are 2 subtypes: ⅢA and ⅢB, ⅢA has 2 varieties, ⅢB has 2 varieties. Harvesting white wild-type and mutant M-0 clustered together in agronomic traits and cluster analysis of SRAP molecular markers. Six biological traits of leaf shape, leaf color, new leaf color, potato color, potato shape and flesh color of wild type and its mutant M-0 were observed and compared. The results showed that both of the five traits No significant difference was observed, but significant differences were observed only in potato color traits, ie white harvest of potato flesh was white while mutant M-0 was pink. Harvesting white mutant M-0 and wild-type polymorphic bands was 29.49%, the number of effective alleles was 1.2085, indicating that the two were not significantly different in genetics, and initially concluded that the harvest of white M-0 is a bumper harvest The result of point mutation in potato flesh gene.