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本文介绍的用配子体培养液感染蚊虫将恶性疟原虫传播正常人体的方法。应用的六名18—31岁的健康男性均无疟疾病史,均无抗疟抗体。根据体内对氯喹的敏感性和对蚊虫的感染力选择恶性疟原虫NF_(54)株进行培养,将培养液与新鲜人血混合,使配子体浓度达1％,膜法喂养弗氏按蚊和斯氏按蚊使其感染。计数喂血7—10天后含囊合子的蚊虫比例和喂血14—17天后涎腺含孢子体的蚊虫比例以评价蚊虫感染率。选择感染率50％以上,涎腺密度2+(11—100个子孢子/每对涎腺)以上的那批蚊子感染志愿者。感染时,将一盛5只蚊子的小笼放在志愿者的臂上,让蚊虫吸血5分钟,吸血后立即
This article describes the method used to infect gametocytes in mosquitoes infected with P. falciparum in normal humans. Six healthy 18- to 31-year-old men who applied had no history of malaria and no anti-malarial antibodies. The Plasmodium falciparum NF_ (54) strain was selected according to its sensitivity to chloroquine and its susceptibility to mosquitoes. The culture medium was mixed with fresh human blood to make the gametophyte concentration reach 1% Anopheles mosquito infection. The proportion of cystic zygotic mosquitoes after 7-10 days of blood feeding and the ratio of mosquito-containing sporozoites in salivary glands after 14-17 days of blood donation were counted to evaluate the mosquito infection rate. Select the infection rate of more than 50%, salivary gland density 2+ (11-100 sporozoites / per salivary gland) above the batch of mosquitoes infected volunteers. Infection, a small cage of 5 mosquitoes placed on the volunteer’s arm, so that mosquitoes vomiting for 5 minutes, immediately after vampire