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目的:探讨分泌性中耳炎(SOM)患者的中耳积液中IFNγ浓度测定的临床意义。方法:用双抗夹心酶联免疫吸附法检测51例(56耳)SOM患者(中耳炎组)的中耳积液、血清及24例正常成人(对照组)血清中IFNγ的浓度。结果:中耳炎组积液中IFNγ的浓度明显高于其血清中的浓度(P<0.01);中耳炎组与对照组血清中的浓度差异无统计学意义(P>0.05);SOM慢性期IFNγ浓度明显高于急性期(P<0.05);首次穿刺及第2次穿刺积液中IFNγ的浓度差异无统计学意义(P>0.05);而第3次或3次以上穿刺积液中IFNγ的浓度则明显升高(P<0.01)。结论:中耳积液中的IFNγ可由中耳腔局部产生,而非单纯由血液中渗透而来;中耳积液中IFNγ的高浓度可作为SOM转为慢性病程或迁延不愈的参考。
Objective: To investigate the clinical significance of IFNγ concentration in middle ear effusion of patients with secretory otitis media (SOM). Methods: The concentrations of IFNγ in serum of middle ear and serum of 24 cases of SOM patients (otitis media group) and serum of 24 normal adults (control group) were detected by double-antibody sandwich enzyme-linked immunosorbent assay. Results: The concentration of IFNγ in effusion of otitis media group was significantly higher than that in serum (P <0.01). There was no significant difference in serum concentration between otitis media group and control group (P> 0.05) (P <0.05). There was no significant difference in the concentrations of IFNγ between the first puncture and the second puncture effusion (P> 0.05). However, the concentration of IFNγ in the third or more puncture effusion was higher than that in the acute phase Was significantly higher (P <0.01). CONCLUSIONS: IFNγ in the middle ear effusion may be produced locally in the middle ear cavity rather than purely from the bloodstream. High concentrations of IFNγ in the middle ear effusions may be used as a reference for the conversion of SOM to a chronic disease or uncontinuation.