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采用平板对峙培养法和滤纸片培养法测定细菌BMY-1菌株对番茄灰霉病菌(Botrytis cinerea)、辣椒疫霉病菌(Phytophthora capsici)、玉米小斑病菌(Bipolaria maydis)、玉米弯孢霉叶斑病菌(Curvularia lunata)等11种植物病原真菌生长的抑制作用。结果表明,BMY-1菌株对供试病原菌生长具有很好的抑制效果。平板对峙培养法中,BMY-1菌株对番茄灰霉病菌、玉米弯孢霉叶斑病菌、玉米小斑病菌的抑制效果最好,抑菌宽度分别达到15.0 mm,14.0 mm,13.0mm。滤纸片培养法中,BMY-1菌液对番茄灰霉病菌抑制效果最好,抑菌带宽度为8.0 mm。BMY-1菌液及其无菌发酵液对病原菌孢子萌发也有很强的抑制作用。其中对辣椒疫霉病菌和玉米小斑病菌孢子萌发的抑制效果最好。BMY-1菌液处理后,孢子萌发率分别为0.97%和1.00%,而对照萌发率为分别为96!29%和93.04%。
Bacterial strain BMY-1 was tested for its resistance to Botrytis cinerea, Phytophthora capsici, Bipolaria maydis, Curvularia lunata by using plate confrontation culture and filter paper culture. (Curvularia lunata) and other 11 kinds of plant pathogenic fungi growth inhibition. The results showed that the strain BMY-1 had a good inhibitory effect on the growth of the tested pathogens. In the plate confrontation culture method, BMY-1 strain had the best inhibitory effect on Botrytis cinerea, Curvularia lunata and Cercospora sojina, and the inhibitory width reached 15.0 mm, 14.0 mm and 13.0 mm respectively. In filter paper culture, BMY-1 had the best inhibitory effect on Botrytis cinerea, with an antimicrobial tape width of 8.0 mm. BMY-1 bacterial liquid and its sterile fermentation broth also have a strong inhibitory effect on spore germination of pathogenic bacteria. Among them, the inhibitory effect on Phytophthora capsici and Spore germination were the best. The spore germination rates were 0.97% and 1.00%, respectively, while the control germination rates were 96.29% and 93.04%, respectively, after treatment with BMY-1.