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目的:研究Fas抗原介导的死亡效应在超抗原诱导T细胞凋亡中的作用。方法:SEA诱导从人外周血建立的短期SEA反应T细胞系凋亡，1.8％琼脂凝胶DNA电泳观察调亡的特征条带;FCM检测Fas、FasL的表达量变化，Fura-2／AM荧光指示剂检测胞浆[Ca2+]的变化。结果:使用FCM特异性检测调亡亚G0／G1蜂大小及1.8％琼脂糖凝胶电泳检测凋亡DNA梯状图谱证明，SEA能诱导短期SEA反应T细胞凋亡，其凋亡的时间与程度与SEA的使用剂量有关。SEA1μg／ml建立的短期SEA反应T细胞对SEA1μg／ml的再次刺激，凋亡量随作用时间的延长而增多，在作用的第16h最高，达58％。FCM间接荧光染色法检测发现，Fas及FasL亦随SEA作用时间的延长而表达增多，16h时表达最多，分别为92％和60％。用Fura-2／AM荧光指示剂检测此时细胞胞浆[Ca2+]已从刺激前的（290±33）nmol／L上升到（680±16）nmol／L。结论:Fas抗原与SEA诱导的T细胞凋亡密切相关，换言之，Fas抗原在超抗原诱导T细胞凋亡中起作用，而胞浆[Ca2+]i升高与SEA诱导的凋亡T细胞DNA断裂及其它凋亡形态变化有关。
AIM: To investigate the role of Fas antigen-mediated death in superantigen-induced apoptosis of T cells. Methods: Apoptosis of T cells induced by short-term SEA reaction was established by SEA in vitro. The characteristic bands of apoptosis were observed by electrophoresis with 1.8% agarose gel electrophoresis. The expressions of Fas and FasL were detected by FCM. Fura-2 / AM fluorescence Indicators to detect changes in cytoplasmic [Ca2 +]. Results: The apoptotic DNA ladder was detected by flow cytometry (FCM), and the apoptotic DNA ladder was analyzed by 1.8% agarose gel electrophoresis. The results showed that SEA can induce the apoptosis of T cells in short-term SEA, the time and degree of apoptosis And the use of SEA dose. SEA1μg / ml established short-term SEA response of T cells SEA1μg / ml of re-stimulation, the amount of apoptosis increased with the duration of action, the highest role in the first 16h, up to 58%. FCM indirect staining showed that the expression of Fas and FasL increased with the prolongation of SEA time and reached the peak at 16h, which were 92% and 60% respectively. The amount of [Ca2 +] in cytoplasm increased from (290 ± 33) nmol / L to (680 ± 16) nmol / L before stimulation with Fura-2 / AM fluorescent indicator. CONCLUSIONS: Fas antigen is closely related to the apoptosis of T cells induced by SEA. In other words, Fas antigen plays a role in the superantigen-induced T cell apoptosis. However, the increase of cytoplasmic [Ca2 +] i is associated with SEA-induced DNA damage of apoptotic T cells And other morphological changes related to apoptosis.