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中国农科院蔬菜所在黄瓜原生质体培养研究方面自1983年获得愈伤组织以来,最近又以原生质体来源的愈伤组织诱导出胚状体。这种胚状体不但产生的量多,同时在继代培养中还可不断形成。胚状体生长速度快,产生数量大,再生植株遗传性状稳定,能避免愈伤化可能产生的倍性和染色体变异。经诱导,目前有的已长出叶片和根。国际上至今尚无关于胚状体成株的报道。他们还用结球甘蓝“报春”的叶肉原生质通过细胞胚胎发生途径培养出完整的植株。甘蓝属芸薹属植物,而芸薹属植物通过原生质体培养获得再生植株是比较困难的。近年来国外多数是用子叶和根为材料获得再生植株,而由叶肉原生质体通过体细胞胚胎发生途径获得完整植株,国内外尚未见报道。
Chinese Academy of Agricultural Sciences cucumber protoplast culture research Since the callus was obtained in 1983, the protoplast derived callus induction embryoid body. Not only does this embryoid body produce more amounts, it also continues to form in the subculture. Embryoid body grows fast, produce a large number of regenerated plants stable genetic trait, can avoid the ploidy and chromosomal variation may occur callus. After induction, some have now grown leaves and roots. There is no report of embryonic body adult in the world so far. They also cultivated the entire plant via the cytoplasmic route using the mesophyll protoplasts of “primula” in Brassica oleracea. Brassica is Brassica, while Brassica is more difficult to obtain regenerated plant by protoplast culture. In recent years, most foreign countries are using cotyledons and roots as materials to obtain regenerated plants, and mesophyll protoplasts obtained through somatic embryogenesis of intact plants, have not been reported at home and abroad.