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目的研究骆驼刺总黄酮最佳提取工艺条件。方法采用正交试验设计研究不同浓度的乙醇、溶媒体积和提取次数对骆驼刺总黄酮回流提取的影响;采用紫外分光光度法测定提取物中总黄酮含量。结果骆驼刺总黄酮最佳提取条件为:即40%乙醇,料液比1:15,提取2次。结论该方法简便、快速、准确,适用于该药材总黄酮的提取工艺研究。
Objective To study the optimum extraction conditions of total flavonoids from Alhagi sparus. Methods The effects of different concentrations of ethanol, solvent volume and extraction times on the extraction of total flavonoids from Alhagi sp. Were studied by orthogonal design. The content of total flavonoids in the extract was determined by UV spectrophotometry. Results The optimum extraction conditions for the total flavonoids of camel thorn: namely 40% ethanol, the ratio of material to liquid was 1:15, extracted twice. Conclusion The method is simple, rapid and accurate and is suitable for the extraction of total flavonoids from this medicine.