Inhibiting effect of CaMK Ⅱ N up-regulation on leukemia cells growth and its mechanism

• 作 者: 侯军
• 机 构: Dept Hematol, Shanghai People's Hosp Affili Tongji Univ,
• 出 处: China Medical Abstracts(Internal Medicine)
• 发表日期: 2014年01期
• 关键词: myeloid empty cytometry inhibited inhibit stained cycle markedly proportion colo

Objective To investigate the inhibitory effects of CaMKⅡN on acute myeloid leukemia cell line HL-60to explore a novel therapeutic target of leukemia.Methods Human CaMKⅡN gene expression vector pcDNA3.1/hCaMKⅡN or empty vector pcDNA3.1/myc-His(-)B was transfected into HL-60 cells by Lipofectamine2000.Human CaMKⅡN proteins of transfected cells were detected by Westem blot.Cell proliferation affected by human CaMKⅡN was determined by MTT.Colonyforming assay was performed by soft agar

Objective To investigate the inhibitory effects of CaMKIIN on acute myeloid leukemia cell line HL-60to explore a novel therapeutic target of leukemia. Methods Human CaMKIIN gene expression vector pcDNA3.1 / hCaMKIIN or empty vector pcDNA3.1 / myc-His (-) B was transfected into HL-60 cells by Lipofectamine 2000. Human CaMK II N proteins of transfected cells were detected by Westem blot. Cell proliferation affected by human CaMK II N was determined by MTT. Colonyforming assay was performed by soft agar