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目的:通过比较基因组学方法对哺乳动物NR3亚基进行分析,探讨其种间功能及结构差异,并预测其功能。方法:应用人类NR3A和NR3B所编码氨基酸序列作为检索探针,通过TBLASTN检索数据库,并应用生物软件进行进化分析及序列对比。结果:共检索到30个哺乳动物NR3A及NR3B基因,其中28个具有完整CDS。进化树显示相同种属均形成独立分支,与人类相似度越高的物种进化距离越近,氨基酸种间比较显示邻接半胱氨酸序列及loop1结构保守性较好,而富含脯氨酸序列在灵长类和啮齿类存在差异。结论:NR3A亚基在人类和啮齿类可能存在功能差异,应用动物模型进行实验应考虑差异可能带来的影响,此外,可推断哺乳动物间NR3亚基富含半胱氨酸序列及loop1结构产生的生理过程及功能相似。
OBJECTIVE: To compare mammalian NR3 subunits with comparative genomics to explore their interspecies functional and structural differences and to predict their function. Methods: The amino acid sequences encoded by human NR3A and NR3B were used as search probes. The databases were searched by TBLASTN. The biological software was used for phylogenetic analysis and sequence comparison. Results: A total of 30 mammalian NR3A and NR3B genes were found, of which 28 had complete CDS. Phylogenetic tree showed that the same species formed an independent branch, the closer the evolutionary distance of the species with higher similarity to human, the comparison of amino acids showed that the contiguous cysteine sequence and loop1 structure were more conservative while the proline rich sequence There are differences in primates and rodents. CONCLUSIONS: NR3A subunits may have functional differences in humans and rodents. Animal models should be used for experiments to consider possible differences. In addition, it is inferred that the NR3 subunit is rich in cysteine residues and loop1 in mammals The physiological processes and functions are similar.