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为了克服恶性疟原虫体外标准培养法中每天更换培养液的麻烦,作者观察了连续培养3天换1次培养液时疟原虫的生长繁殖情况。所用虫种为已经在体外培养6个月的科摩罗群岛FCPS25株恶性疟原虫。开始培养时,将含虫血用培养液制成50%混悬液,原虫率分为0.25%和0.05%两组,培养皿直径皆为35mm,每个培养皿各加0.2ml含虫血混悬液,然后再分别加入含有35mMHEPES和10%人血清的RPMI 1640培养液1.5、2.25、3、3.75 ml,连续培养3天,中间不换培养液,比较二组疟原虫的繁殖情况。结果在开始培养时原虫率为0.25%组中,各培养皿
In order to overcome the troubles of replacing the culture medium daily with P. falciparum standard culture method, the author observed the growth and reproduction of the parasite when the culture medium was changed continuously for three days. The insect species used was Comoros FCPS25 Plasmodium falciparum, which had been cultured in vitro for 6 months. At the beginning of culture, the worm-blood-containing culture medium was made into a 50% suspension, and the parasite rate was divided into two groups of 0.25% and 0.05%. The diameter of the dish was 35 mm. Each dish was added with 0.2 ml of worm- The suspension was added with 1.5, 1.25, 3 and 3.75 ml of RPMI 1640 medium containing 35 mM HEPES and 10% human serum, respectively. The culture was continued for 3 days without any change of culture medium. The reproduction of the two groups of malaria parasite was compared. Results At the beginning of culture, the parasite rate was 0.25% of the plates in each dish