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目的:探讨双氢青蒿素(DHA)对鼻咽癌CNE-2细胞的生长抑制作用及其机制。方法:以体外培养的CNE-2细胞为研究对象,分别采用CCK-8法观察不同浓度和时间DHA对CNE-2细胞增殖的抑制作用,流式细胞仪Annexin V-FITC法检测CNE-2细胞的凋亡率,caspase-3活性检测法检测不同浓度DHA处理后CNE-2细胞中caspase-3的活性变化。结果:CCK-8法显示,与对照组相比,随DHA浓度的增加,CNE-2细胞的增殖显著抑制(P<0.05);流式细胞仪Annexin V-FITC法,结果显示DHA诱导CNE-2细胞凋亡具有浓度依赖性;caspase-3活性检测法显示,与对照组相比,随DHA浓度的增加,caspase-3的活性显著提高(P<0.05)。结论:DHA能有效抑制CNE-2细胞的增殖并诱导其凋亡,其机制可能是能上调CNE-2细胞中caspase-3的活性。
Objective: To investigate the inhibitory effect of dihydroartemisinin (DHA) on the growth of CNE-2 cells and its mechanism. Methods: The CNE-2 cells cultured in vitro were studied. The inhibitory effect of DHA with different concentration and time on the proliferation of CNE-2 cells was observed by CCK-8 assay. The expression of CNE-2 cells was detected by Annexin V-FITC The activity of caspase-3 in CNE-2 cells treated with different concentrations of DHA was detected by caspase-3 activity assay. Results: Compared with the control group, the proliferation of CNE-2 cells was significantly inhibited by CCK-8 assay (P <0.05). The flow cytometry Annexin V-FITC method showed that DHA induced CNE- 2 cell apoptosis in a concentration-dependent manner. Compared with the control group, the caspase-3 activity assay showed that caspase-3 activity was significantly increased with the increase of DHA concentration (P <0.05). CONCLUSION: DHA can effectively inhibit the proliferation of CNE-2 cells and induce its apoptosis. The mechanism may be that it can up-regulate the activity of caspase-3 in CNE-2 cells.