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用液相法合成了ＡＣＴＨ４～１０片段，并对７位氨基酸残基进行替换，获得２个类似物．从Ｃ端逐步接肽时，３５ｍｏｌ／Ｌ氯化氢乙酸乙酯溶液可有效地脱去叔丁氧羰基保护基，偶联反应用ＤＣＣ作缩合剂，合成２肽至４肽需Ｈｏｂｔ活化，合成５肽至７肽若用Ｈｏｂｔ活化可导致收率降低．该方法可用于类似片段的大量合成．
ACTH4 ~ 10 fragments were synthesized by liquid phase method, and the 7 amino acid residues were replaced to obtain two analogues. From the C-terminal peptide, 3 5mol / L hydrogen chloride ethyl acetate solution can effectively remove the tert-butoxycarbonyl protecting group coupling reaction using DCC as a condensing agent to synthesize peptide 2 to 4 peptides to be Hobt activation, The activation of Hobt with synthetic 5-peptide to 7-peptide results in a decrease in yield. This method can be used for mass synthesis of similar fragments.