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目的探讨亚低温对Nogo-A和生长相关蛋白(GAP-43)表达的影响,为亚低温的临床应用提供理论依据。方法将实验动物分为正常对照组、亚低温干预组、室温恢复组,应用免疫组化方法检测标本中Nogo-A和GAP-43蛋白,比较各组标本阳性细胞的个数,初步探讨亚低温对Nogo-A和GAP-43表达的影响。结果亚低温干预组与室温恢复组相比,qNogo-A(Ⅲ,Ⅱ)=8.83,qgap-43(Ⅱ,Ⅲ)=11.65,差异均有统计学意义(P<0.01)。而室温恢复组又与正常对照组相比,qNogo-A(Ⅲ,Ⅰ)=11.16,qgap-43(Ⅰ,Ⅲ)=13.98,差异也均有统计学意义(P<0.01)。结论亚低温干预可抑制Nogo-A的表达,维持接近正常的GAP-43表达,有利于新生动物缺氧缺血性脑损伤早期神经元的再生。
Objective To investigate the effects of mild hypothermia on the expression of Nogo-A and growth-associated protein (GAP-43), and to provide a theoretical basis for the clinical application of mild hypothermia. Methods The experimental animals were divided into normal control group, mild hypothermia intervention group and room temperature recovery group. Immunohistochemistry was used to detect the expression of Nogo-A and GAP-43 protein in each group. The numbers of positive cells in each group were compared. On the expression of Nogo-A and GAP-43. Results Compared with room temperature recovery group, qNogo-A (Ⅲ, Ⅱ) = 8.83, qgap-43 (Ⅱ, Ⅲ) = 11.65, the difference was statistically significant (P <0.01). Compared with the normal control group, qNogo-A (Ⅲ, Ⅰ) = 11.16 and qgap-43 (Ⅰ, Ⅲ) = 13.98 in the room temperature recovery group were also significantly different (P <0.01). Conclusion Mild hypothermia can inhibit the expression of Nogo-A and maintain the normal expression of GAP-43, which is beneficial to the regeneration of neurons in the early stage of neonatal hypoxic-ischemic brain damage.