【摘 要】
：
利用G四聚体可以熄灭荧光的特性以及T-Hg2+-T的特殊结构,发展了一种简便的T on”型碘离子检测新方法.设计了一条5端标有荧光基团的富T序列,3端采用能形成G四聚体的富G序列代
【机 构】
：
湖南大学化学生物传感与计量学国家重点实验室,化学化工学院,生物纳米与分子工程湖南省重点实验室,长沙410082;
论文部分内容阅读
利用G四聚体可以熄灭荧光的特性以及T-Hg2+-T的特殊结构,发展了一种简便的T on”型碘离子检测新方法.设计了一条5端标有荧光基团的富T序列,3端采用能形成G四聚体的富G序列代替传统的熄灭基团.加入汞离子后,富T序列形成T-Hg2+-T机构发生折叠,G四聚体靠近荧光基团,发生光诱导电子转移,使荧光被熄灭.若加入碘离子,碘离子会与汞离子形成较稳定的配合物,汞离子从DNA上被竞争下来,探针的荧光得以恢复,且荧光强度与50 ～ 500 nmol/L的碘离子呈良好线性关系,检出限为30 nmol/L.本方法选择性好,10倍于碘离子浓度的其它常见阴离子干扰较小.检测自来水样中碘离子的回收率为92％～ 109％,相对标准偏差RSD＜4％(n=4).
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