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目的:观察复方菊明对大鼠离体胸主动脉环收缩作用的影响。方法:采用离体血管张力实验法,观察复方菊明、维拉帕米(Ver)和生理盐水对NA、高K+液引起的主动脉环收缩的影响,以及对NA诱导的主动脉环依赖细胞内钙与外钙收缩的影响。结果:复方菊明(4.0mg/ml~8.0mg/ml)和Ver能拮抗NA(2.37μmol/l)和KCl(2.37μmol/l)诱导的主动脉环收缩作用,生理盐水无影响。在相同的浓度下,复方菊明拮抗NA诱导的主动脉环收缩作用比对KCl诱导的主动脉环收缩的拮抗作用强。此外,复方菊明(5.0mg/ml)对NA诱导的依赖细胞内钙、外钙的收缩均有抑制,Ver对NA诱导的依赖细胞内钙的收缩有抑制作用、对依赖细胞外钙的收缩没有影响;生理盐水组给药前后没有变化。结论:复方菊明具有直接舒张血管作用,能阻断受体依赖性钙通道(ROC)和电压依赖性钙通道(VDC),其降压机制可能通过阻断ROC,抑制内钙释放外钙内流,从而降低胞内Ca2+浓度,发挥降压作用。
Objective: To observe the effects of Fufang Juguang injection on contraction of isolated rat aorta rings. Methods: The effects of compound jingming, verapamil (Ver) and normal saline on aortic ring contraction induced by NA and high K + fluid were observed by means of in vitro vascular tension test, and the effect of NAA-induced aortic ring-dependent cells Effect of calcium and external calcium contraction. Results: The effects of Fufang Juming (4.0mg / ml ~ 8.0mg / ml) and Ver can antagonize the contraction of aortic rings induced by NA (2.37μmol / L) and KCl (2.37μmol / At the same concentration, compound chrysanthemum antagonized NA-induced contraction of the aortic rings more strongly than KCl-induced contraction of the aortic rings. In addition, compound pyrethrin (5.0mg / ml) inhibited NA-induced contraction of intracellular calcium and extracellular calcium, and Ver inhibited NA-induced contraction of intracellular calcium and inhibited extracellular calcium-dependent contraction No effect; saline group before and after administration did not change. CONCLUSION: Fufang juaming has a direct vasodilatory effect and can block receptor-dependent calcium channel (ROC) and voltage-dependent calcium channel (VDC). Its antihypertensive mechanism may be through blocking ROC and inhibiting calcium release Flow, thereby reducing intracellular Ca2 concentration, play a role in antihypertensive.