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通过建立多粘菌素ＢＳｕｂ— ＭＩＣｓ诱导大肠杆菌形成溶菌环的实验模型，对自溶环上细菌进行了动态观察，以光学显微镜检查，可见随着培养时间延长，细菌淡染程度加重；以活菌计数法观察到活菌数量的下降；用透射电镜观察到细菌胞壁不完整或全部破损，内容物流失，确证了多粘菌素 Ｂ Ｓｕｂ—ＭＩＣｓ诱导大肠杆菌形成的溶菌环上细菌发生了自溶。根据Ｈｉｇｇｉｎｓ细菌胞壁合成模型和流出泵原理，讨论了细菌在抗生素ＳＵｂ—ＭＩＣｓ作用下发生自溶的机制，提示抗生素Ｓｕｂ－ＭＩＣｓ引起细菌发生的自溶反应可能在临床微生物学校验中有其应用价值。
Through the establishment of polymyxin BSub-MICs induced E. coli formation of the bacteriolysis model of the bacteria on the autolysis loop was observed by light microscopy, showing that as the incubation time, the degree of bacterial contamination increased; to live The number of viable cells was decreased by the bacterial counting method. The cell wall was incomplete or damaged by transmission electron microscopy, and the contents were lost. It was confirmed that bacteria on the bacteriolytic ring of E.coli induced by polymyxin B Sub-MICs Autolysis. According to the Higgins bacterial cell wall synthesis model and the outflow pump principle, the mechanism of autolysis of bacteria under the action of the antibiotic SUb-MICs is discussed, suggesting that antibiotic Sub-MICs may cause bacterial autolytic reactions that may have applications in clinical microbiological verification value.