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目的:建立HPLC-ELSD法测定肾八味胶囊中黄芪甲苷的含量。方法:色谱柱:Hypersil SB-C_(18)柱(250 mm×4.6 mm,5μm);柱温:室温;流动相:乙腈:水(32:68);流速:1 ml·min~(-1);蒸发光散射检测器参数:漂移管温度:105℃;雾化温度:50℃;载气:氮气(0.99999%),流量:2.0 L·min~(-1)。结果:黄芪甲苷的线性范围为0.25～8.00μg,r=0.999 6;平均回收率为98.3%,RSD为2.0%(n=6)。结论:本方法简便,结果准确可靠,可作为控制肾八味胶囊质量的方法。
Objective: To establish HPLC-ELSD method for the determination of astragaloside IV in Renweibawei capsule. Methods: Column: Hypersil SB-C_(18) column (250 mm×4.6 mm, 5 μm); column temperature: room temperature; mobile phase: acetonitrile: water (32:68); flow rate: 1 ml·min~(-1) Evaporative light scattering detector parameters: drift tube temperature: 105 °C; atomization temperature: 50 °C; carrier gas: nitrogen (0.99999%), flow rate: 2.0 L · min ~ (-1). Results: The linear range of Astragaloside IV was 0.25-8.00μg, r=0.999 6. The average recovery was 98.3% and the RSD was 2.0% (n=6). Conclusion: The method is simple, accurate and reliable, and can be used as a method to control the quality of Shenbawei capsules.