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目的:优化超声-回流联用提取黄连-白术药对中主要有效成分小檗碱型生物碱的条件.方法:以乙醇为溶剂,利用响应面分析法考察超声提取温度、超声功率、超声时间、乙醇浓度、黄连与乙醇料液比例、回流提取次数对黄连-白术药对中小檗碱型生物碱提取效果的影响.结果:得出其最佳提取条件为将黄连、白术细粉浸泡于1∶35倍量的60％的乙醇中,置于47℃、40％功率下的恒温超声仪中提取40 min,再回流提取3次,得到的提取液小檗碱型生物碱提取率最高(30.96％).结论:得到的多元回归模型的准确性和适用性较好,可以用该模型来分析和预测黄连-白术药对中小檗碱型生物碱的超声-回流提取工艺.“,”Objective:To study the optimal conditions of berberine with ultrasonic-reflux extraction from Rhizoma Coptidis and Atractylodes.Methods:Alcohol was used as dissolvent.Response surface method (RSM)was used to study effects of ultrasonic temperature,ultrasonic power,ultrasonic time,ethanol concentration,solid-liquid ratio,reflux extraction times on the extraction yield of berberine.Results:The optimal conditions of berberine were as follows:solid-liquid ratio 1 ∶ 35,ethanol concentration 60％,ultrasonic temperature 47 ℃,ultrasonic power 40％,ultrasonic time 40 min,reflux extraction frequency 3 times.Under the optimal conditions,the extraction rate of berberine reaches 30.96％.Conclusion:Then the quadric polynomial regression model was established,and can be used to analyze and predict the extraction process of berberine from Rhizoma Coptidis and Atractylodes with ultrasonic-reflux extraction.