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以贴梗海棠[Chaenomeles speciosa(Sweet)Nakai]不同花色的24份种质为材料,调查了其花色在CIE L*a*b*表色系的分布状况,利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和高效液相色谱-电喷雾离子化-多级质谱联用技术(HPLC-ESI-MSn)定性定量分析其花青苷组分,运用多元线性回归方法分析了花色与花青苷组成之间的关系。结果表明,贴梗海棠花瓣中共含有6种花青苷,分别是矢车菊素-3-O-半乳糖苷(Cy3Ga)、矢车菊素-3-O-葡萄糖苷、天竺葵素-3-O-半乳糖苷、天竺葵素-3-O-(半乳糖葡萄糖苷)[Pg3(Ga-G)]、矢车菊素-3-O-(半乳糖葡萄糖苷)以及矢车菊素-3-O-琥珀酸-阿拉伯糖苷(Cy3SucAra)。Cy3SucAra为首次发现。其中,Cy3Ga、Pg3(Ga-G)和Cy3SucAra是决定贴梗海棠花色的主要色素,这3种色素含量的增加导致花色显著变红。基于花青苷的组成信息,探讨了贴梗海棠的花色改良和蓝色花创制的策略。
Twenty-four germplasm of different color of Chaenomeles speciosa (Sweet) Nakai were used as materials to investigate the distribution of their flower color in the CIE L * a * b * colorimetric system. High performance liquid chromatography-photodiode array (HPLC-ESI-MSn) was used to qualitatively and quantitatively analyze the anthocyanin components of the anthocyanins. HPLC-ESI-MSn was used to analyze the components of anthocyanin. The multivariate linear regression was used to analyze the color and flower The relationship between the composition of green glycosides. The results showed that the total petals contained 6 kinds of anthocyanins, which were cyanidin-3-O-galactoside (Cy3Ga), cyanidin-3-O-glucoside, pelargonidin-3-O - galactoside, pelargonidin-3-O- (galactoside) [Pg3 (Ga-G)], cyanidin-3-O- (galactoside) O-succinic-arabinoside (Cy3 SucAra). Cy3SucAra was first discovered. Among them, Cy3Ga, Pg3 (Ga-G) and Cy3SucAra are the main pigments that determine the flower color of Panax Begonia. The increase of these three kinds of pigment results in a significant reddening of flower color. Based on the composition of anthocyanin, the paper discussed the improvement of flower color and the strategy of blue flower creation.