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发现１８种氨基酸特别是色氨酸．在ＴｏｙｏｐｅａｒｌＨＷ－４０Ｓ树脂所填色谱柱上表现出非理想排阻行为的基础上，以色氨酸在该柱上的保留时间为指标，探讨不同流动相改良剂对该凝胶与色氨酸之间的相互作用，阐明这种反常行为是疏水作用，离子交换以及氢键三者综合作用的结果，而疏水作用是最主要的影响因素．实验结果表明，流动相组成对该凝胶色谱行为具有明显的影响，从而有助于确立该凝胶理想的排阻色谱条件，为利用该凝胶所具有的吸附性能分离分析肽类和氨基酸等小分子物质奠定了基础
18 kinds of amino acids were found, especially tryptophan. Based on the non-ideal exclusion behavior of Toyopearl HW-40S resin column, the retention time of tryptophan on the column was taken as an index to investigate the effect of different mobile phase modifiers on the gel and tryptophan The interaction between the two groups shows that this abnormal behavior is the result of the combination of hydrophobic interaction, ion exchange and hydrogen bonding. Hydrophobic interaction is the most important factor. The experimental results show that the composition of the mobile phase has obvious influence on the chromatographic behavior of the gel, thus helping to establish the ideal exclusion chromatographic conditions for the gel. In order to utilize the adsorption properties of the gel to separate peptides and amino acids Small molecule material laid the foundation