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目的建立大鼠心脏移植超急性排斥反应的实验动物模型。方法以BN大鼠为供者,Lewis大鼠为受者。供、受者间连续进行3次皮肤移植,使受者预致敏。再进行颈部异位心脏移植。采用微量淋巴毒试验监测受者体内抗供者抗体滴度的变化。结果预致敏后的受者体内抗供者抗体滴度明显升高。7只受者心脏移植后有6只移植心在24h内被排斥,病理学证实为超急性排斥反应。结论供、受者间连续3次皮肤移植预致敏后,再行心脏移植可以建立稳定的同种移植超急性排斥反应模型。
Objective To establish an animal model of hyperacute rejection in rat heart transplantation. Methods BN rats as donors, Lewis rats as recipients. Donor, recipient 3 consecutive skin transplants, the recipient pre-sensitized. Re-neck ectopic heart transplantation. The amount of anti-donor antibody titers in the recipients was monitored using a lymphatic toxicity test. The results of pre-sensitized recipients in vivo anti-donor antibody titers increased significantly. Six of the seven recipients after heart transplantation were rejected within 24 hours and were pathologically confirmed as hyperacute rejection. Conclusions After donor skin grafts were sensitized three times in succession, cardiac allograft could establish a stable allograft model of hyperacute rejection.