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目的建立鉴别常用藏药二十五味大汤丸和测定羟基红花黄色素A的方法。方法采用薄层色谱法和高效液相色谱法鉴定和测定。结果二十五味大汤丸中没食子酸、角茴香、木香、藏木香和乌奴龙胆均能通过薄层色谱进行鉴别,羟基红花黄色素A含量的回收率为99%～105%。结论所用方法可用于控制二十五味大汤丸的质量。
Objective To establish a method to identify common Tibetan medicine Twenty-five Wei Da Tang pill and determine hydroxysafflor yellow A. Methods TLC and HPLC were used for identification and determination. Results Twenty-five Wei Da Tang pill gallic acid, horn fennel, woody, Tibetan wood and Ukrainian dragon gall can be identified by thin-layer chromatography, the recovery of hydroxysafflor yellow A content of 99% to 105 %. Conclusion The method used can be used to control the quality of Twenty-five Wei Da Tang pill.