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Objective:To determine the effects of different cytokinins at various concentrations onin vitro shoot multiplication of an important medicinal plant.Methods:Nodal explants(1.5-2.0 cm)of Sophora tonkinensiswere used.Multiple shoots were induced from nodal explants cultured onthe Murashige and Skoog(MS)medium supplemented with 0.0,0.5,1.0,2.0,4.0,8.0,or 16.0μmol2-isopentyladenine(2iP),N6 benzyladenine,kinetin or thiadiazuron.Results:Among the fourinvestigated cytokinins,2iP showed the best response for shoot multiplication.Maximum shootinduction(75%)was achieved on the MS medium supplemented with 2.0μmol 2iP,with a meannumber of 5.0 shoots per explant.In comparison to other cytokinins tried,2iP showed the highestshoot elongation with a mean shoot length of 4.8 cm.Root initiation was observed within 15 dwithin the transfer of shoots onto the MS basal medium,and the rooting percentage was 100%with a mean number of 5.4 roots per shoot and root length of 6.2 cm over a period of 4 weeks.Thehealthy plants,hardened and transferred to a greenhouse for proper acclimatization,exhibited100%survival.Conclusions:It can be summarized that 2iP is the optimal plant growth regulatorforSophoramultiplication.
Objective: To determine the effects of different cytokinins at various concentrations on in vitro shoot multiplication of an important medicinal plant. Methods: Nodal explants (1.5-2.0 cm) of Sophora tonkinensiswere used. Multiple shoots were induced from nodal explants cultured on the Murashige and Skoog MS) supplemented with 0.0, 0.5, 1.0, 2.0, 4.0, 8.0, or 16.0 μιηοΐ of 2-isopentyladenine (2iP), N6 benzyladenine, kinetin or thiadiazuron.Results: Among the four investigated cellsokinins, 2iP showed the best response for shoot multiplication. shootinduction (75%) was achieved on the MS medium supplemented with 2.0 μmol 2iP, with a mean number of 5.0 shoots per explant.In comparison to other cytokinins tried, 2iP showed the highestshoot elongation with a mean shoot length of 4.8 cm. Root initiation was observed within 15 dwithin the transfer of shoots onto the MS basal medium, and the rooting percentage was 100% with a mean number of 5.4 roots per shoot and root length of 6.2 cm over a period of 4 weeks. hardened and transferred to a greenhouse for proper acclimatization, labeled 100% survival. Conclusions: It can be summarized that 2iP is the optimal plant growth regulator for Sophoramultiplication.