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目的:筛查验证前列腺癌特异性表达基因。方法:运用基因芯片筛查出前列腺癌组织和癌旁组织中差异表达的基因,再通过PCR验证。结果:从芯片结果中发现,总共有1 444个基因(差异倍数≥1.5;P≤0.05)为差异表达基因。其中,前列腺癌与对比配对的良性组织有769个上调(53%)和675个下调(47%)。差异基因中有40%的差异倍数为1.5~2倍,包括396个上调和182个下调基因。另外308个上调基因和334个下调基因的差异倍数为2~5倍;46个上调基因和78个下调基因的差异倍数为5~10倍;19个上调基因和81个下调基因的差异倍数为10倍以上。取其中上调和下调最明显的各15个基因做进一步荧光定量PCR(qRT-PCR)鉴定,结果显示了大多数基因都有芯片结果相似的基因片段,芯片结果和qRT-PCR结果用皮尔森相关性分析结果为0.83,并获得10个差异显著的基因。结论:芯片分析出来的前列腺癌和良性组织间差异基因是可靠的,qRT-PCR验证获得的这10个差异基因可能成为新的肿瘤标记物和特征性肿瘤鉴定分子。
Objective: Screening for prostate cancer-specific expression of genes. Methods: Genes differentially expressed in prostate cancer tissues and adjacent tissues were screened by gene chip and confirmed by PCR. Results: From the chip results, a total of 1 444 genes (with a multiple of ≥ 1.5; P ≤ 0.05) were differentially expressed genes. Among them, there were 769 up-regulated (53%) and 675 down-regulated (47%) prostate cancer and paired benign tissues. Differences in 40% of the genes in the fold of 1.5 to 2 times, including 396 up-regulated and 182 down-regulated genes. In addition, the difference between 308 up-regulated genes and 334 down-regulated genes was 2 to 5 times; the difference between 46 up-regulated genes and 78 down-regulated genes was 5 to 10 times; the difference between 19 up-regulated genes and 81 down-regulated genes was 10 times more. QRT-PCR was used to identify the 15 genes with the most significant up-regulation and down-regulation. The results showed that most of the genes had similar gene chip results. The chip results and qRT-PCR results were correlated with Pearson The result of sex analysis was 0.83, and 10 genes with significant difference were obtained. Conclusion: The differentially expressed genes between prostate cancer and benign tissues are reliable. The 10 differentially expressed genes confirmed by qRT-PCR may become new tumor markers and characteristic tumor identification molecules.