Bcl-2基因及其家族Bax,Bcl-Xl和Fas/Apo-l,P16的检测在急性白血病中的临床意义

• 作 者: 陈燕 周剑锋 乐蓓蓓 向直富 宋晓华 李崇渔 王辨明
• 出 处: 癌症
• 发表日期: 1998年05期
• 关键词: Bcl-2基因 Bcl-Xl P16 白血病发生 细胞凋亡 蛋白印迹 抗原表达 统计差异 急性髓性白血病

目的：为了解白血病凋亡正、负反馈网络中的问题与临床的关系，研究了抑制凋亡的基因Ｂｃｌ２及其家族Ｂａｘ，ＢｃｌＸｌ以及诱导凋亡的基因Ｆａｓ／Ａｐｏ１，Ｐ１６。方法：采用细胞免疫组化，ＷｅｓｔｅｒｎＢｌｏｔ，以及ＮｏｒｔｈｅｒｎＢｌｏｔ的方法。结果：发现在ＡＭＬ组和ＡＬＬ组，Ｂｃｌ２抗原表达均明显高于正常（Ｐ＜００１），回顾性分析ＡＭＬＣＲ组其明显低于ＮＲ组（Ｐ＜００１）。蛋白印迹ＣＲ组Ｂｃｌ２表达虽然降低，但和ＮＲ组比较无统计学意义（Ｐ＞００５）。Ｂｃｌ２ｍＲＮＡ的表达ＣＲ组明显低于ＮＲ组（Ｐ＜０．０１）。Ｂａｘ的表达在免疫组化中白血病明显低于正常（Ｐ＜００１），但在ＣＲ组与ＮＲ组，免疫组化，蛋白印迹，ＢａｘｍＲＮＡ的表达，均无差异（Ｐ＞００５）。但ＢｃｌＸｌｍＲＮＡ的表达两组间有明显差异（Ｐ＜００１）。Ｆａｓ／Ａｐｏ１的表达为白血病组低于正常（Ｐ＜００１），但在ＣＲ与ＮＲ组中，免疫组化与蛋白印迹分析均无统计差异（Ｐ＞００５）。Ｐ１６的表达为白血病组低于正常组（Ｐ＜００１），但在ＣＲ组与ＮＲ组中无统计学意义（Ｐ＞００５）。结论：Ｂｃｌ２抗原以及Ｂｃｌ２ｍＲＮＡ，Ｂｃｌ?

OBJECTIVE: To understand the relationship between clinical problems and positive and negative feedback networks in leukemia apoptosis, the gene Bcl-2, which inhibits apoptosis, and its family Bax, Bcl-Xl, and Fas/Apo-1, an apoptosis-inducing gene, were studied. P16. METHODS: Cell immunohistochemistry, WesternBlot, and NorthernBlot methods were used. Results: The expression of Bcl2 antigen was significantly higher in the AML group and the ALL group than in the normal group (P<001). The retrospective analysis of the AMLCR group was significantly lower than that of the NR group (P<001). Although the expression of Bcl-2 in the western blot group was lower than that in the NR group, it was not statistically significant (P>005). The expression of Bcl2 mRNA in CR group was significantly lower than that in NR group (P<0.01). Bax expression was significantly lower than normal in immunohistochemistry (P<001), but there was no difference in immunohistochemistry, Western blot, and Bax mRNA expression between CR and NR groups (P>005). . However, the expression of BclX1 mRNA was significantly different between the two groups (P<001). The expression of Fas/Apo  1 was lower than normal in the leukemia group (P<001), but there was no statistical difference between immunohistochemistry and Western blot analysis in the CR and NR groups (P>005). The expression of P16 was lower in the leukemia group than in the normal group (P<001), but it was not statistically significant in the CR group and the NR group (P>005). Conclusion: Bcl2 antigen and Bcl2 mRNA, Bcl?