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目的:建立测定加味苓桂术甘汤中甘草次酸的HPLC法。方法:选用Diamonsil C18柱(4.6mm×150mm,5μm)为色谱柱,流动相为甲醇-水-醋酸铵(83∶16∶1);流速0.8mL·min-1;柱温:35℃;波长254nm。结果:用外标法定量分析,线性范围为13.29～66.45mg·mL-1,r=0.99999;精密度RSD为0.32%;平均加样回收率为100.03%,RSD为2.12%(n=6);测得加味苓桂术甘汤中甘草次酸的含量为25.316mg·mL-1。结论:本法操作简单、快捷,结果准确可靠,可作为有效检测加味苓桂术甘汤中甘草次酸含量的方法。
Objective: To establish a HPLC method for determination of glycyrrhetinic acid in modified Lingguizhugan decoction. Methods: The Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) was used as the mobile phase. The mobile phase was methanol-water-ammonium acetate (83:16:1), the flow rate was 0.8 mL · min- 254nm. Results: The linear range was 13.29 ~ 66.45 mg · mL-1, r = 0.99999. The RSD of precision was 0.32%. The average recovery was 100.03% with RSD of 2.12% (n = 6) The content of glycyrrhetinic acid in Modified Lingguizhugan Decoction was 25.316mg · mL-1. Conclusion: This method is simple, fast, accurate and reliable, and can be used as an effective method to detect the contents of glycyrrhetinic acid in flavored Linggui Shugan Decoction.