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本研究旨在观察急性应激刺激对癫痫模型大鼠慢性期癫痫发作的影响。首先建立了氯化锂(LiCl)-匹罗卡品(pilocarpine,PILO)癫痫大鼠模型,在其慢性自发反复发作期给予猫尿和足部电刺激类急性应激刺激,检测应激刺激前后癫痫模型大鼠的行为学表现以及癫痫发作行为。结果显示,癫痫模型大鼠在猫尿刺激之后自发活动明显减少,危险评估活动增多,但是连续观察45min无癫痫发作。给予癫痫模型鼠足部电刺激也未诱发其癫痫发作。与此相反,与对照组相比,腹腔注射低剂量的戊四氮(pentylenetetrazole,PTZ,30mg/kg),在癫痫模型鼠更容易诱发癫痫发作,而且其发作时间也明显延长。以上结果表明猫尿或者足部电刺激虽然导致癫痫模型大鼠的应激行为,但是不足以在短时间内诱发其癫痫发作。
The purpose of this study was to investigate the effects of acute stress stimulation on chronic epileptic seizures in epileptic rats. First, a lithium chloride (pilocarpine, PILO) epilepsy rat model was established. The rats were given acute stress stimulation of cat urine and foot electrical stimulation during chronic spontaneous recurrent episodes. Behavior of epilepsy model rats and seizure behavior. The results showed that the spontaneous activity of epilepsy model rats decreased significantly after cat litter irritation, and the risk assessment activities increased, but no epileptic seizures were observed continuously for 45 minutes. The epilepsy model rats given foot electrical stimulation did not induce their seizures. In contrast, intraperitoneal injection of pentylenetetrazole (PTZ, 30 mg / kg) intraperitoneally induced epileptic seizures more easily in epilepsy model rats, and the onset time was significantly longer. The above results show that cat urine or foot electrical stimulation, although causing the stress behavior of epilepsy rats, is not sufficient to induce seizures in a short time.