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自Parker和Geer等(1960)分别应用电镜证明家兔实验性动脉粥样硬化及人主动脉粥样硬化斑块内的泡沫细胞来自平滑肌细胞以来,用培养的动脉平滑肌细胞进行动脉粥样硬化研究者愈来愈多。国内关于平滑肌细胞培养虽有报道,但涉及超微结构方面仍欠仔细。作者应用单层细胞超薄切片法,对培养的家兔主动脉平滑肌细胞的超微结构进行立体的观察,以供参考。材料和方法取4周龄体重600克的新西兰白兔的胸主动脉进行实验。原代培养用组织块种植法,动物用乙醚麻醉致死,在无菌条件下提取出胸主动脉。在放大镜下用大头针将其一端固定在无菌的硅橡胶板上,小心地将外膜剥净,然后沿纵轴剪开,继用大头针固定其四角,使内膜平展开来。先用磷
Since Parker and Geer et al. (1960) used electron microscopy to demonstrate that atherosclerotic and foam cells in human aortic atherosclerotic plaques originate from smooth muscle cells in rabbits, the atherosclerotic study of cultured arterial smooth muscle cells More and more people. Domestic reports on the smooth muscle cell culture, but the ultrastructure is still concerned about the details. The authors applied the monolayer cell ultrathin section method to observe the ultrastructure of cultured rabbit aortic smooth muscle cells for reference. Materials and Methods The thoracic aorta of 4-week-old New Zealand white rabbits weighing 600 g was used for the experiment. Tissue culture was used for primary culture. Animals were anesthetized with ether and thoracic aorta was extracted under aseptic conditions. Under the magnifying glass with a pin to one end fixed in a sterile silicone rubber sheet, carefully peel the outer membrane, and then cut along the longitudinal axis, followed by pins fixed its corners, the flattening of the intima. First with phosphorus