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目的:观察雷公藤片对实验性自身免疫性甲状腺炎(EAT)小鼠甲状腺激素、自身抗体、一氧化氮含量的影响及作用机制。方法:猪甲状腺球蛋白与完全弗氏佐剂混合免疫小鼠,收集脾淋巴细胞作小鼠尾静脉注射,第6周处死后收集脾淋巴细胞。放射免疫法检测甲状腺功能与自身抗体;Griess氏反应测上清液一氧化氮含量。结果:模型组甲状腺功能略高于正常对照组,与各治疗组比较均无统计学意义。EAT模型组TGA、TMA水平明显高于正常组(P<0.01)。模型组NO量高于正常对照组(P<0.01),雷公藤片可明显抑制NO的产生。结论:采用猪甲状腺球蛋白致敏的淋巴细胞可成功免疫诱导出小鼠实验性自身免疫性甲状腺炎。雷公藤片可降低甲状腺自身抗体水平,对EAT小鼠在细胞免疫、体液免疫方面的紊乱有纠正作用,可抑制甲状腺过强的自身免疫反应。
Objective: To observe the effects of Tripterygium tablets on thyroid hormone, autoantibodies and nitric oxide in experimental autoimmune thyroiditis (EAT) mice and its mechanism. Methods: Mice were immunized with porcine thyroglobulin and complete Freund’s adjuvant. Splenic lymphocytes were collected and injected into tail vein of mice. After the sixth week, spleen lymphocytes were collected. Thyroid function and autoantibodies were detected by radioimmunoassay. The content of nitric oxide in supernatant was measured by Griess reaction. Results: The thyroid function of the model group was slightly higher than that of the normal control group, and there was no significant difference between the two groups. The levels of TGA and TMA in EAT model group were significantly higher than those in normal group (P <0.01). The amount of NO in model group was higher than that in normal control group (P <0.01). Tripterygium wilfordii tablet could obviously inhibit the production of NO. Conclusion: Experimental thyroiditis can be induced by lymphocytes primed by porcine thyroglobulin. Tripterygium tablets can reduce thyroid autoantibodies, EAT mice in cellular immunity, humoral immunity disorders have a role in correcting, can inhibit the thyroid too strong autoimmune response.