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目的:初步探讨丙酮酸脱氢酶复合物的E2亚单位(PDCE2)与原发性胆汁性肝硬化(PBC)发病机制间的关系.方法:用纯化的PDCE2重组蛋白体外刺激PBC患者外周血单个核细胞(PBMCs);3HTdR掺入法检测PBMCs增殖情况;ELASA法检测细胞上清中TNFα含量.结果:11例M2抗体阳性和1例AMA阳性的PBC患者中有8例出现不同程度PBMCs增殖,而2例AMA和M2抗体均阴性的PBC患者和4例AIH患者PBMCs均无增殖.11例M2抗体阳性的PBC患者刺激后TNFα分泌均明显增加.结论:PBC患者外周血中存在针对PDCE2的自身反应性T细胞;TNFα可能在PBC的病理损伤中发挥重要作用.
OBJECTIVE: To investigate the relationship between the E2 subunit of pyruvate dehydrogenase complex (PDCE2) and the pathogenesis of primary biliary cirrhosis (PBC) .Methods: Purified PDCE2 recombinant protein was used to stimulate peripheral blood mononuclear cells The proliferation of PBMCs was detected by 3HTdR incorporation method and the levels of TNFα in the supernatant of cells were detected by ELASA method.Results: 8 cases of PBMCs positive in 11 cases of M2 antibody and 1 case of AMA positive cells showed different degrees of PBMC proliferation, However, no PBMCs were found in 2 PBC patients with negative AMA and M2 antibody and 4 with AIH, and the secretion of TNFα increased in 11 patients with M2 antibody positive PBC.Conclusion: Reactive T cells; TNFα may play an important role in the pathological damage of PBC.