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In this paper, a two-scale finite element approach is proposed and analyzed for approxi-mations of Green’s function in three-dimensions. This approach is based on a two-scale finite element space defined, respectively, on the whole domain with size H and on some subdomain containing singu-lar points with size h (h << H). It is shown that this two-scale discretization approach is very efficient. In particular, the two-scale discretization approach is applied to solve Poisson-Boltzmann equations successfully.