Rapid Amplification of 5′ cDNA End of S. Liaotungensis Choline Monooxygenase Using Inverse PCR RACE

• 作 者: 李秋莉 Gao Xiaorong FAN Qi
• 机 构: Bioengineering Department of Dalian University of Technology, Biology Department of Liaoning Normal
• 出 处: 高技术通讯
• 发表日期: 2004年期
• 关键词: Inverse PCR Rapid amplification of cDNA ends S. Lianotungenesis Choline monooxyg

Based on part of a known cDNA sequence of Suaeda Liaotungensis choline monooxygenase, the authors successfully cloned the 5′ cDNA end of Suaeda Lianotungensis choline monooxygenase using Inverse PCR RACE with a specially designed 5′-phosphated RT primer and two pairs of specific inverse PCR primers. Compared with the anchored PCR RACE, inverse PCR RACE has better specificity and higher amplification.