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目的:观察大肠癌区域引流淋巴结中CD4+CD25+调节性T细胞(Treg)情况及其与白细胞介素10(IL-10)和转化生长因子β1(TGF-β1)水平的关系。方法:用免疫组化法检测大肠癌患者(淋巴结转移32例,无转移28例)区域引流淋巴结中CD4+CD25+的表达(Treg细胞数目);ELISA法检测外周血血清IL-10和TGF-β1水平;分析Treg数目与IL-10和TGF-β1水平的相关性。结果:CD4+CD25+在淋巴结转移患者阳性淋巴结中呈高表达,阴性淋巴结中呈低表达,而在无转移患者淋巴结中几乎无表达;淋巴结转移患者阳性淋巴结CD4+CD25+的OD值明显高于阴性淋巴结与无转移患者淋巴结(P=0.012;P=0.002),转移患者的阴性淋巴结与无转移患者淋巴结OD值差异未达统计学意义(P=0.115)。淋巴结转移患者外周血血清IL-10,TGF-β1水平均明显高于无转移患者(P=0.003;P=0.004)。患者淋巴结CD4+CD25+的OD值与血清IL-10或TGF-β1浓度之间均呈明显正相关(r=0.683,P=0.000;r=0.532,P=0.000)。结论:大肠癌患者区域引流淋巴结中Treg细胞水平与淋巴结转移密切相关,血清IL-10或TGF-β1水平可作为监测淋巴结转移的指标。
Objective: To investigate the relationship between CD4 + CD25 + regulatory T cells (Treg) and interleukin-10 (IL-10) and transforming growth factor-β1 (TGF-β1) in draining lymph nodes of colorectal cancer. Methods: The expression of CD4 + CD25 + (Treg cells) in regional lymph node was detected by immunohistochemistry in 32 cases of colorectal cancer (lymph node metastasis, 28 cases without metastasis). The levels of IL-10 and TGF-β1 Level; analyze the correlation between the number of Tregs and the levels of IL-10 and TGF-β1. Results: CD4 + CD25 + was highly expressed in lymph node positive lymph nodes, negative lymph nodes, and almost no lymph node metastasis in patients with lymph node metastasis. The positive rate of CD4 + CD25 + in patients with lymph node metastasis was significantly higher than that in negative lymph nodes There was no significant difference in lymph node OD value between lymph node metastasis and non-metastatic lymph node metastasis (P = 0.012; P = 0.002) (P = 0.115). Serum levels of IL-10 and TGF-β1 in patients with lymph node metastasis were significantly higher than those without metastasis (P = 0.003; P = 0.004). There was a significant positive correlation between OD value of CD4 + CD25 + and serum IL-10 or TGF-β1 (r = 0.683, P = 0.000; r = 0.532, P = 0.000). Conclusion: The level of Treg cells in regional draining lymph nodes in patients with colorectal cancer is closely related to lymph node metastasis. Serum levels of IL-10 or TGF-β1 may be used as indicators to monitor lymph node metastasis.